Composite

Part:BBa_M41006:Design

Designed by: Hao Xing   Group: MIT BE 20.20-S13   (2013-05-12)


Hydrocarbon Sensor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4274
    Illegal SpeI site found at 4643
    Illegal PstI site found at 2764
    Illegal PstI site found at 3903
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1679
    Illegal SpeI site found at 4643
    Illegal PstI site found at 2764
    Illegal PstI site found at 3903
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4516
    Illegal BglII site found at 6633
    Illegal XhoI site found at 392
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4274
    Illegal SpeI site found at 4643
    Illegal PstI site found at 2764
    Illegal PstI site found at 3903
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4274
    Illegal SpeI site found at 4643
    Illegal PstI site found at 2764
    Illegal PstI site found at 3903
    Illegal NgoMIV site found at 3269
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3431
    Illegal BsaI.rc site found at 2902
    Illegal BsaI.rc site found at 3204


Design Notes

This sensor is specific for our system. The output can be changed by removing LacI gene and adding other gene.


Source

Canosa, I., et al. "A Positive Feedback Mechanism Controls Expression of AlkS, the Transcriptional Regulator of the Pseudomonas Oleovorans Alkane Degradation Pathway." Molecular microbiology 35.4 (2000): 791-9. Van Beilen, JB, et al. "Analysis of Pseudomonas Putida Alkane-Degradation Gene Clusters and Flanking Insertion Sequences: Evolution and Regulation of the Alk Genes." Microbiology 147.6 (2001): 1621-30.

References